Efek Gel Kentang Kuning (Solanum tuberosum L.) terhadap Proses Penyembuhan Luka pada Mencit (Mus musculus)

Silvana Anggreini Rosa, Sudigdo Adi, Achadiyani Achadiyani, Astrid Feinisa Khairani, Uci Ary Lantika

Abstract


Perawatan luka yang baik diperlukan dalam proses penyembuhan luka. Salah satu metodenya adalah pemberian obat topikal. Gel kentang kuning (Solanum tuberosum L.) memiliki kandungan antosianin yang berperan dalam meningkatkan vaskularisasi, menginisiasi sintesis DNA, dan menstimulus sintesis fibronektin dari fibroblas. Dengan demikian, dimungkinkan gel kentang kuning dapat membantu proses penyembuhan luka. Penelitian ini bertujuan melihat efek gel kentang kuning pada jumlah fibroblas, tebal epitel, dan luas luka eksisi. Penelitian ini merupakan eksperimental laboratorik dengan rancangan acak lengkap yang dilakukan di kandang hewan Divisi Biologi Sel Departemen Anatomi, Fisiologi, dan Biologi Sel, Fakultas Kedokteran, Universitas Padjadjaran; Laboratorium Patologi Anatomi, Universitas Padjadjaran; dan Laboratorium Farmasi Singaperbangsa, Universitas Padjadjaran, Bandung. Penelitian dilakukan pada bulan Mei sampai Oktober 2015. Tiga puluh enam mencit (Mus musculus) jantan galur Swiss Webster dieksisi pada kulitnya kemudian dibagi menjadi dua kelompok: kelompok perlakuan (n=18) dan kelompok kontrol (n=18). Dilakukan pengamatan luas luka dan histologi pada hari ke-7, 14, dan 25. Dibuat sediaan preparat histologi untuk menghitung jumlah fibroblas, pembuluh darah, dan tebal epitel. Hasil penelitian memperlihatkan bahwa pemberian gel kentang kuning dapat meningkatkan efektivitas pembentukan fibroblas dan pembuluh darah pada hari ke-7. Selain itu, gel kentang kuning juga berefek pada peningkatan tebal epitel dan penurunan diameter luas luka pada hari ke-7, 14, dan 25. Simpulan, pemberian gel kentang kuning dapat meningkatkan efektivitas penyembuhan luka eksisi.

 

THE EFFECT OF YELLOW POTATO (SOLANUM TUBEROSUM L.) GEL ON WOUND HEALING PROCESS IN MICE (MUS MUSCULUS)

Adequate wound care is needed on wound-healing process. Applying topical agent is one of the wound care methods. Yellow potato (Solanum tuberosum L.) gel’s content an anotsianin antioxidant that could improve vascularization, initiation DNA synthesis, and stimulate synthesis of fibronectin. Therefore, it is possible that yellow potato gel could help on wound healing process. This study examined the effect of yellow potato gel on wound healing. This study was laboratory experiment with completely randomized design conducted in Department of Anatomy, Physiology and Cell Biology, Faculty of Medicine, Universitas Padjadjaran; Anatomical Pathology Laboratory, Universitas Padjadjaran; and Singaperbangsa Pharmacy Laboratory, Universitas Padjadjaran, Bandung. The study was conducted from May to October 2015. Thirty six male Swiss Webster mice (Mus musculus) were divided into 2 groups: the experimental group, which received a topical application of yellow potato gel and the control group without gel application. The observationsscar width and histological were conducted on days 7, 14, and 25. Histological preparation was made to calculate the fibroblasts, blood vessels, and epithelial thickness. The result of this study showed that topical application of the yellow potato gel evidently increased effectiveness of fibroblasts and blood vessels development on days 7. More over, it was also shown improvement in epithelial thickness and scar width on days 7, 14, and 25. In conclusion, yellow potato gel treatment can improve the effectiveness of wound healing


Keywords


Blood vessel; epitel; epithelial; fibroblas; fibroblast; gel kentang kuning; pembuluh darah; yellow potato gel

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DOI: https://doi.org/10.29313/gmhc.v6i1.2417

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