Soyghurt Supernatant on Mouse Embryonic Fibroblast (MEF) Cell

Uci Ary Lantika, Astrid Feinisa Khairani


Yogurt is a functional food developed with various modifications in the fermentation process. Replacing animal milk into soymilk as raw material is one approach. Yogurt has a good effect on human health. Probiotic and bioactive compounds in yogurt can inhibit cell proliferation and stimulate apoptosis on the cancer cell line. However, there is no report about the effect of yogurt on a normal cell. This research was conducted to examine the impact of soyghurt supernatant intervention toward the viability of mouse embryonic fibroblast (MEF) cell. The is an in vitro study using MEF cell isolated from 10th days gestational age mice embryo conducted at Microbiology Laboratorium and Cell Culture and Cytogenetic Laboratory, Universitas Padjadjaran, Bandung in November 2018–January 2019. Soyghurt made from soymilk fermented by Lactobacillus bulgaricus ATCC 11842. The number of the bacterial colony calculated by total plate count (TPC) method and pH calculated by pH meter. Soyghurt supernatant was made from soyghurt and then intervened into MEF cells by 1–20% concentration. The cell viability showed in the 50% inhibitory concentration (IC50) analysis. The intervention of soyghurt supernatant at 1–20% concentration showed there was no proliferation inhibition until 50% population (IC50). However, from the morphology analysis, there was MEF cell morphology alteration on the group given soyghurt supernatant with >12.5% concentration. Counter mechanism effect from soymilk fermentation by probiotic could be the driver for this result. In conclusion, soyghurt supernatant intervention at 1–20% concentration did not have a cytotoxic effect on MEF cell, but enhancement of soyghurt supernatant concentration can increase cytotoxic potential.



Yoghurt merupakan functional food yang dikembangkan dengan berbagai modifikasi dalam proses pembuatannya. Mengganti susu hewan dengan susu kedelai sebagai bahan baku adalah salah satunya. Yoghurt memiliki efek yang baik bagi kesehatan manusia. Senyawa probiotik dan bioaktif pada yoghurt dapat menginhibisi proliferasi sel dan menstimulasi apoptosis pada sel lini kanker. Akan tetapi, tidak terdapat laporan mengenai efek yoghurt pada sel normal. Penelitian ini dilakukan untuk menguji pengaruh intervensi supernatan soyghurt terhadap viabilitas sel mouse embryonic fibroblast (MEF). Ini adalah penelitian in vitro menggunakan sel MEF yang diisolasi dari embrio tikus hari ke-10 usia kebuntingan yang dilakukan di Laboratorium Mikrobiologi dan Laboratorium Kultur Sel dan Sitogenetika, Universitas Padjadjaran, Bandung pada November 2018–Januari 2019. Soyghurt dibuat dari susu kedelai yang difermentasi oleh Lactobacillus bulgaricus ATCC 11842. Jumlah koloni bakteri dihitung dengan metode total plate count (TPC) dan pH diukur dengan pH meter. Supernatan soyghurt dibuat dari soyghurt dan kemudian diintervensi ke dalam sel MEF dengan konsentrasi 1–20%. Viabilitas sel ditunjukkan dalam analisis penghambatan 50% (IC50). Pemberian supernatan soyghurt konsentrasi 1–20% menunjukkan tidak terdapat inhibisi proliferasi 50% (IC50). Namun, dari analisis morfologi, terdapat perubahan morfologi sel MEF pada kelompok yang diberi supernatan soyghurt dengan konsentrasi >12,5%. Efek mekanisme yang saling meniadakan dari fermentasi susu kedelai dengan probiotik diduga menjadi mekanisme hasil dari penelitian ini. Simpulan, intervensi supernatan soyghurt pada konsentrasi 1–20% tidak memiliki efek sitotoksik pada sel MEF, namun peningkatan konsentrasi supernatan soyghurt dapat meningkatkan potensi sitotoksik.


Cell viability; mouse embryonic fibroblast (MEF); supernatan soyghurt; soyghurt supernatan; viabilitas sel

Full Text:



Fakri EFM, Lim SM, Musa NH, Hasan MH, Adam A, Ramasamy K. Lactobacillus fermentum LAB 9-fermented soymilk with enriched isoflavones and antioxidants improved memory in vivo. Sains Malays. 2016;45(9):1289–97.

Nurhajati J, Sayuti, Chrysanti, Syachroni. An in-vitro model for studying the adhesion of Lactobacillus bulgaricus in soyghurt and enteropathogenic Escherichia coli (EPEC) on HEp-2 cells. Afr J Microbiol Res. 2012;6(24):5142–6.

Vioretti R, Khairani AF, Fauziah PN, Hilmanto D. An evaluation of soyghurt potential on tumor necrosis factor-α and soluble endoglin levels in preeclampsia maternal serum-induced placental trophoblast cell in vitro. Int Food Res J. 2018;25(4):1397–402.

Williamson C. Functional foods: what are the benefits? Br J Community Nurs. 2009;14(6):230–6.

Malik SS, Saeed A, Baig M, Asif N, Masood N, Yasmin A. Anticarcinogenecity of microbiota and probiotics in breast cancer. Int J Food Prop. 2018;21(1):655–66.

Douglas CC, Johnson SA, Arjmandi BH. Soy and its isoflavones: the truth behind the science in breast cancer. Anticancer Agents Med Chem. 2013;13(8):1178–87.

Puranik SI, Ghagane SC, Nerli RB, Jalalpure SS, Hiremath MB. Evaluation of in vitro antioxidant and anticancer activity of Simarouba glauca leaf extracts on T-24 bladder cancer cell line. Phcog J. 2017;9(6):906–12.

Srivastava S, Somasagara RR, Hegde M, Nishana M, Tadi SK, Srivastava M, et al. Quercetin, a natural flavonoid interacts with DNA, arrests cell cycle and causes tumor regression by activating mitochondrial pathway of apoptosis. Sci Rep. 2016;6:24049.

Ghagane SC, Puranik SI, Kumbar VM, Nerli RB, Jalalpure SS, Hiremat MB, et al. In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines. Integr Med Res. 2017;6(1):79–87.

Yunita M, Hendrawan Y, Yulianingsih R. Analisis kuantitatif mikrobiologi pada makanan penerbangan (aerofood ACS) Garuda Indonesia berdasarkan TPC (total plate count) dengan metode pour plate. JKPTB. 2015;3(3):237–48.

Yeganegi M, Watson CS, Martins A, Kim SO, Reid G, Challis JR, et al. Effect of Lactobacillus rhamnosus GR-1 supernatant and fetal sex on lipopolysaccharide-induced cytokine and prostaglandin-regulating enzymes in human placental trophoblast cells: implications for treatment of bacterial vaginosis and prevention of preterm labor. Am J Obstet Gynecol. 2009;200(5):532.e1–8.

Durkin ME, Qian X, Popescu NC, Lowy DR. Isolation of mouse embryo fibroblasts. Bio Protoc. 2013;3(18):e908.

Chen ZY, Hsieh YM, Huang CC, Tsai CC. Inhibitory effects of probiotic Lactobacillus on the growth of human colonic carcinoma cell line HT-29. Molecules. 2017;22(1):E107.

Bahuguna A, Khan I, Bajpai VK, Kang SC. MTT assay to evaluate the cytotoxic potential of a drug. Bangladesh J Farmacol. 2017;12(2):115–8.

Senthilraja P, Kathiresan K. In vitro cytotoxicity MTT assay in vero, HepG2 and MCF-7 cell lines study of marine yeast. J Appl Pharm Sci. 2015;5(3):080–4.

Božanić R, Lovković S, Jeličić I. Optimizing fermentation of soymilk with probiotic bacteria. Czech J food Sci. 2011;29(1):51–6.

Kregiel D, Berlowska J, Witonska I, Antolak H, Proestos C, Babic M, et al. Saponin-based, biological-active surfactants from plants. In: Najjar R, editor. Application and characterization of surfactants. Rijeka, Croatia: InTech; 2017. p. 183–205.

Lorent JH, Quetin-Leclercq J, Mingeot-Leclercq M. The amphilic nature of saponins and their effects on artificial and biological membranes and potential consequences for red blood and cancer cells. Org Biomol Chem. 2014;12(44):8803–22.

Ahuja A, Kim JH, Kim JH, Yi YS, Cho JY. Functional role of ginseng-derived compounds in cancer. J Ginseng Res. 2018;42(3):248–54.

Si Y, Zhu J, Huang X, Zhu P, Xie C. Effects of Panax notoginseng saponins on proliferation and differentiation of rat embryonic cortical neural stem cells. J Chin Med Assoc. 2016;79(5):256–63.

Park JH, Lee SK, Kim MK, Lee JH, Yun BH, Park JH, et al. Saponin estracts induced apoptosis of endometrial cells from women with endometriosis through modulation of miR-21-5p. Reproductive Sci. 2018;25: 292–301.

Awouafack MD, Tane P, Kuete V, Eloff JN. Sesquiterpenes from medicinal plants of Africa. In: Kuete V, editor. Medicinal plant research in Africa: pharmacology and chemistry. London, UK: Elsevier; 2013. p. 33–103.

Chadwick M, Trewin H, Gawthrop F, Wagstaff C. Review Sesquiterpenoids lactones: benefits to plants and people. Int J Mol Sci. 2013;14(6):12780–805..

Nurulita NA, Kusuma AM, Darsini, Delvia W, Yulianti VT. The cytoprotective and cell recovery properties of apple extracts on H2O2 induced-NIH3T3 cells: an anti aging candidate. Indones J Cancer Chemoprevent. 2018;9(2):78–85.

Wakkary JJ, Durry M, Kairupan C. Pengaruh pemberian getah bonggol pisang (Musa paradisiaca var. sapientum L. Kuntze. AAB) terhadap penyembuhan luka sayat pada kulit tikus Wistar (Rattus novergicus). eBM. 2017;5(1):15018.

Weremfo A, Adinortey MB, Pappoe ANM. Haemostatic effect of the stem juice of Musa paradisiaca L. (Musaceae) in guinea pigs. Advan Biol Res. 2011;5(4):190–2.

Klotoé JR, Dougnon TV, Sacramento TI, Dandjesso C, Edorh AP, Koudokpon H, et al. Hemostatic potential of the sap of Musa sapientum L. (Musaceae). J Appl Pharm Sci. 2012;2(6):65–9.


pISSN 2301-9123 | eISSN 2460-5441

Visitor since 19 October 2016:

View My Stats

Free counters!

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.