Acute Toxicity Test of Unripe Papaya (Carica papaya L.) Aqueous Extract (UPAE) on The Blood Urea and Creatinine Concentration

Yuktiana Kharisma, Yuke Andriane, Titik Respati

Abstract


Unripe papaya aqueous extract (UPAE) widely used as lactation stimulator, anti-diabetes, anti-bacterial, and anti-inflammatory. The utilization of papaya is not known for its safety yet, so it is necessary to research its toxicity. The purpose of this study was to investigate the acute toxicity of UPAE on renal function through measurement of blood urea and creatinine levels. This study used pure in vivo experimental design on 11 Swiss Webster mice using the dose of acute toxicity determination based on new recommended methods of 0, 50, 200, 400, 800, 1,000, 1,500, 2,000, 3,000, 4,000, and 5,000 mg/kg body weight (BW). After 24 hours, 1 ml blood drawn through the tail examined for blood urea and creatinine levels. The measurement of urea content using kinetic method point and creatinine level using modified Jaffe method. Provision of UPAE at doses of 0, 50; 200; 400; 800; 1,000 mg/ Kg BW resulted on blood urea equal to 39; 35; 48; 49; 48; 32 mg/dl respectively. Blood urea level 23; 22; 28; 34; 35 mg / dl was obtained at  1,500 UPAE doses; 2,000; 3,000; 4,000; 5.000 mg / Kg BW dosages respectively. After 24 hours of UPAE administration, the  creatinine level  in various doses using new recommended method  of (0-5.000 mg / Kg BW) were 0.75; 0.54; 0.53; 0.50; 0.60; 0.54; 0.52; 0.55; 0.42; 0.51; 0.40 mg / dl. The conclusion of this study was that administration of UPAE to the highest dose of 5,000 mg/kg BW did not cause acute toxicity.

 

TOKSISITAS AKUT EKSTRAK AIR BUAH PEPAYA (CARICA PAPAYA L.) TERHADAP KADAR UREUM DAN KREATININ DARAH

Ekstrak air buah pepaya muda  (EABPM) digunakan secara empiris sebagai laktagogum, antidiabetes, antibakteri, dan antiinflamasi. Tingkat keamanan nya belum banyak diketahui, sehingga perlu dilakukan penelitian uji toksisitas akut. Tujuan penelitian ini adalah mengetahui toksisitas akut EABPM terhadap fungsi ginjal melalui pengukuran kadar ureum dan kreatinin plasma. Penelitian ini menggunakan disain eksperimental murni invivo terhadap 11 ekor mencit betina galur Swiss Webster dengan penentuan dosis sesuai dengan new recommended method: 0, 50, 200, 400, 800, 1.000, 1.500, 2.000, 3.000, 4.000, dan 5.000 mg/kgBB. Setelah 24 jam, diambil darah melalui ekor mencit sebanyak 1 ml untuk diperiksa kadar ureum dan kreatinin plasma. Pengukuran kadar ureum menggunakan point kinetic method dan kadar kreatinin menggunakan metode Jaffe yang dimodifikasi. Pemberian EABPM pada dosis 0, 50; 200; 400; 800; 1.000 mg/KgBB didapatkan  kadar ureum plasma 39; 35; 48; 49; 48; 32 mg/dl secara berurutan. Kadar ureum plasma 23; 22; 28; 34; 35 mg/dl didapatkan pada pemberian dosis EABPM sebanyak 1.500; 2.000; 3.000; 4.000; 5.000 mg/KgBB. Kadar kreatinin plasma dalam berbagai dosis (0-5.000 mg/KgBB) adalah 0,75; 0,54; 0,53; 0,50; 0,60; 0,54; 0,52; 0,55; 0,42; 0,51; 0,40 mg/dl. Simpulannya adalah EABPM tidak menimbulkan tanda toksisitas akut pada fungsi ginjal mencit melalui pengukuran kadar ureum dan kreatinin plasma.


Keywords


Acute toxicity; ekstrak air buah papaya muda; toksisitas akut; unripe papaya aqueous extract

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DOI: https://doi.org/10.29313/gmhc.v6i2.3794

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