Halal Critical Point Analysis of Bajakah Wood (Spatholobus littoralis Hassk.) Nano Particle as Anticancer Agent

Lelly Yuniarti, Yuktiana Kharisma, Titik Respati, Maya Tejasari

Abstract


Bajakah wood contains phenolic compounds, flavonoids, tannins, and saponins with anticancer activity. The discovery and development of new drugs require several stages. In the process, there are many possibilities of adding other substances to form new active substances or as solvents that allow drug preparations to be doubtful of halalness. Hence, it is necessary to analyze the critical point of halal ingredients. The purpose of this study was to determine the characteristics of the nanoparticles of bajakah wood (Spatholobus littoralis Hassk.) and to test the anticancer activity in several cancer cell cultures, as well as to analyze the critical point of halalness of the material. This research method is a composition test using chromatography and anticancer activity test using MTT. Analysis of the critical point of halal materials using hazard analysis critical control point (HACCP). The research was carried out at the Indonesian Engineering Nanotechnology Laboratory South Tangerang and the UGM Integrated Laboratory Sleman in July–December 2020. The results showed that bajakah wood nanoparticles contained pure water as a solvent, viscosity 0.08878 cP, scattering intensity 1.1059 cps, diameter 176.1+/−43.7 (nm). Cytotoxic test results showed IC50 against cell culture MCF7 1,063.28 (±114.98) g/mL, HepG2 53.34 (±0.35) g/mL, T47D 150.63 (±8.44) g/mL, WiDR 114.38 (±7.82) μg/mL, HTB 97.50 (±3.49) μg/mL, HeLa 182.95 (±36.22) μg/mL, and Vero 710.10 (±106.46) μg/mL. This study concludes that bajakah wood nanoparticles are not critical in terms of halal ingredients. At the same time, their anticancer activity is weak against breast cancer and uterine cervical cancer, medium categories against liver cancer and lung cancer, and is not toxic to normal cells.

 

ANALISIS TITIK KRITIS KEHALALAN NANOPARTIKEL KAYU BAJAKAH (SPATHOLOBUS LITTORALIS HASSK.) SEBAGAI AGEN ANTIKANKER

Kayu bajakah mengandung senyawa fenolik, flavonoid, tanin, dan saponin yang memiliki aktivitas antikanker. Penemuan dan pengembangan obat baru memerlukan beberapa tahapan. Dalam prosesnya terdapat banyak kemungkinan penambahan zat lain untuk membentuk zat aktif baru atau sebagai pelarut yang memungkinkan sediaan obat diragukan kehalalannya. Oleh sebab itu, perlu dilakukan analisis titik kritis kehalalan bahan. Tujuan penelitian ini mengetahui karakteristik sediaan nanopartikel kayu bajakah (Spatholobus littoralis Hassk.) dan menguji aktivitas antikanker pada beberapa kultur sel kanker, serta menganalisis titik kritis kehalalan bahan. Metode penelitian ini adalah uji komposisi menggunakan kromatografi dan uji aktivitas antikanker menggunakan MTT. Analisis titik kritis kehalalan bahan menggunakan hazard analysis critical control point (HACCP). Penelitian dilakukan di Laboratorium Nanovasi Rekayasa Indonesia Tangerang Selatan dan Laboratorium Terpadu UGM Sleman pada Juli–Desember 2020. Hasil penelitian menunjukkan sediaan nanopartikel kayu bajakah mengandung pelarut air murni, viskositas 0,08878 cP, scattering intensity 1,1059 cps, berdiameter 176,1+/−43,7 (nm). Hasil uji sitotoksik menunjukkan IC50 terhadap kultur sel MCF7 1.063,28 (±114,98) μg/mL, HepG2 53,34 (±0,35) μg/mL, T47D 150,63 (±8,44) μg/mL, WiDR 114,38 (±7,82) μg/mL, HTB 97,50 (±3,49) μg/mL, HeLa 182,95 (±36,22) μg/mL, dan Vero 710,10 (±106,46) μg/mL. Kesimpulan penelitian ini bahwa nanopartikel kayu bajakah bersifat tidak kritis dalam kehalalan bahan. Selain itu, aktivitas antikankernya lemah terhadap kanker payudara dan kanker serviks uteri, sedang terhadap kanker hati dan kanker paru, serta tidak toksik pada sel normal.


Keywords


Anticancer; antikanker; bajakah wood; critical halal point; kayu bajakah; nanoparticle; nanopartikel; titik kritis kehalalan

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References


Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424.

Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646–74.

Hedvat M, Emdad L, K Das SK, Kim K, Dasgupta S, Thomas S, et al. Selected approaches for rational drug design and high throughput screening to identify anti-cancer molecules. Anticancer Agents Med Chem. 2012;12(9):1143–55.

Solomon VR, Lee H. Anti-breast cancer activity of heteroaryl chalcone derivatives. Biomed Pharmacother. 2012;66(3):213–20.

Noorlaili, Saputra MMA, Kumalasari E. Uji aktivitas antibakteri ekstrak batang bajakah tampala (Spatholobus littoralis Hassk) terhadap pertumbuhan bakteri Staphylacoccus aureus [undergraduate thesis]. Banjarmasin: STIKES ISFI; 2019 [cited 2020 November 15]. Available from: http://repository.akfar-isfibjm.ac.id/810.

Aziz NA, Majdina H, Hassan Y, Zulkifly HH, Wahab MSA, Aziz MSA, et al. Assessment of the halal status of respiratory pharmaceutical products in a hospital. Proc Soc Behav Sci. 2014;121:158–65.

International Agency for Research on Cancer (IARC). Latest world cancer statistics: global cancer burden rises to 14.1 million new cases in 2012: marked increase in breast cancers must be addressed. Press Release No. 223 [Internet]. 2013 December 12 [cited 2020 November 15]. Available from: https://www.iarc.fr/wp-content/uploads/2018/07/pr223_E.pdf.

American Cancer Society. Cancer facts & figures 2017. Atlanta: American Cancer Society; 2017 [cited 2020 November 15]. Available from: https://www.cancer.org/content/dam/cancer-org/research/cancer-facts-and-statistics/annual-cancer-facts-and-figures/2017/cancer-facts-and-figures-2017.pdf.

Ratanamaneichat C, Rakkarn S. Quality assurance development of halal food products for export to Indonesia. Proc Soc Behav Sci. 2013;88:134–41.

Shield N. Halal food industry: a global outlook. Paper presented at 11th ASEAN Food Conference; Bandar Seri Begawan, Brunei Darussalam; 2009 October 21–23.

Prabowo S, Rahman AA, Rahman SA, Samah AA. Revealing factors hindering halal certification in East Kalimantan Indonesia. J Islam Mark. 2015;6(2):268–91.

Wilson JAJ, Liu J. The challenges of Islamic branding: navigating emotions and halal. J Islam Mark. 2011;2(1):28–42.

Daulay SS. Hazard analysis critical control point (HACCP) dan implementasinya dalam industri pangan [Internet]. Jakarta: Kementerian Perindustrian Republik Indonesia; 2015 [cited 2020 November 15]. Available from: https://kemenperin.go.id/download/6761/HACCP-dan-Implementasinya-Dalam-Industri-Pangan.

Ninkaew S, Chantaranothai P. The genus Spatholobus Hassk. (Leguminosae-Papilionoideae) in Thailand. Trop Nat Hist. 2014;14(2):87–99.

Herianto, Kusuma Z, Nihayati E, Prayogo C. The plant wisdom of Dayak Ot Danum, Central Kalimantan. JTLS. 2018;8(2):130–43.

Saputra MMA, Ayuchecaria N. Uji efektivitas ekstrak etanolik batang bajakah tampala (Spatholobus littoralis Hassk.) terhadap waktu penyembuhan luka. J Ilm Ibnu Sina. 2018;3(2):318–27.

Sattler KD. Handbook of nanophysics: nanoparticles and quantum dots. Boca Raton: CRC Press; 2016.

Rodríguez JA, Fernández-García M. Synthesis, properties, and applications of oxide nanomaterials. Hoboken: John Wiley & Sons; 2007.

Inggriani AS, Husni P. Formulasi nanopartikel untuk terapi kanker. Farmaka. 2016;14(1):127–35.

Amri S, Jamil M, Ardiansyah. Analisis yuridis kewenangan Majelis Ulama Indonesia dalam penyelenggaraan jaminan produk halal. As-Sâis. 2017;5(5):114–32.

Hijriawati M, Putriana NA, Husni P. Upaya farmasis dalam implementasi UU No. 33 Tahun 2014 tentang Jaminan Produk Halal. Farmaka. 2018;16(1):127–32.

Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, et al. Cell viability assays. 2013 May 1 [Updated 2016 Jul 1]. In: Markossian S, Grossman A, Brimacombe K, Arkin M, Auld D, Austin CP, et al., editors. Assay guidance manual [Internet]. Bethesda: Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004 [cited 2020 November 15]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK144065/pdf/Bookshelf_NBK144065.pdf.

van Meerloo J, Kaspers GJL, Cloos J. Cell sensitivity assays: the MTT assay. Methods Mol Biol. 2011;731:237–45.

Baharum Z, Akim AM, Taufiq-Yap YH, Hamid RA, Kasran R. In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao. Molecules. 2014;19(11):18317–31.

Caamal-Fuentes EE, Peraza-Sánchez SR, Torres-Tapia LW, Moo-Puc RE. Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant. Molecules. 2015;20(8):13563–74.

Chahar MK, Sharma N, Dobhal MP, Joshi YC. Flavonoids: a versatile source of anticancer drugs. Pharmacogn Rev. 2011;5(9):1–12.

Bribi N. Pharmacological activity of alkaloids: a review. Asian J Bot. 2018;1(2):1–6.

Heleno SA, Martins A, Queiroz MJRP, Ferreira ICFR. Bioactivity of phenolic acids: metabolites versus parent compounds: a review. Food Chem. 2015;173:501–13.

Sytar O, Hemmerich I, Zivcak M, Rauh C, Brestic M. Comparative analysis of bioactive phenolic compounds composition from 26 medicinal plants. Saudi J Biol Sci. 2018;25(4):631–41.




DOI: https://doi.org/10.29313/gmhc.v9i2.6997

pISSN 2301-9123 | eISSN 2460-5441


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