Activity of Javanese Ginger, Turmeric, Garlic, and Pomegranate Flower on LDL-C and Total-C on Dyslipidemia Model Rats

Larissa Larissa, Penny Setyawati Martioso, Diana Krisanti Jasaputra

Abstract


High levels of LDL cholesterol are risk factors for coronary heart disease. Different types of medicinal plants have hypolipidemic effects. The study aimed to compare the potential of Javanese ginger ethanol extract, turmeric, garlic, and pomegranate flowers with rosuvastatin on levels of LDL cholesterol (LDL-C) and total cholesterol (total-C) male Wistar rats dyslipidemia models. This experimental laboratory research was conducted in Maranatha Animal Research Laboratory Bandung and was carried out in January–December 2020. The experimental animals were divided into six groups (n=5): the control group, the Javanese ginger group, the turmeric group, the garlic group, the pomegranate flower group, and the comparison control group. The induction given to experimental animals was administering vitamin D3, a high-fat diet, and propylthiouracil for 14 days. The results showed that the administration of 175 mg/kg BW of garlic ethanol extract (−44.85%), pomegranate flowers (−58.74%), and rosuvastatin (−40.00%) reduced LDL-C compared to control (p<0.05). The administration of 175 mg/kg BW of Javanese ginger ethanol extract (−15.16%), turmeric (−14.02%), garlic (−22.80%), pomegranate flower (−65.24%), and rosuvastatin (−18.70%) reduced total-C compared to controls (p<0.05). The conclusion is that garlic and pomegranate flowers lowered LDL-C, while Javanese ginger, turmeric, garlic, and pomegranate flowers reduced total-C.

 

AKTIVITAS TEMULAWAK, KUNYIT, BAWANG PUTIH, DAN BUNGA DELIMA TERHADAP K-LDL DAN K-TOTAL PADA TIKUS MODEL DISLIPIDEMIA

Kadar kolesterol LDL yang tinggi adalah faktor risiko penyakit jantung koroner. Berbagai jenis tanaman obat memiliki efek hipolipidemik. Penelitian ini bertujuan membandingkan potensi ekstrak etanol temulawak, kunyit, bawang putih, dan bunga delima dengan rosuvastatin pada kadar kolesterol LDL (K-LDL) dan kolesterol total (K-total) tikus Wistar jantan model dislipidemia. Penelitian laboratorium eksperimental ini dilakukan di Laboratorium Penelitian Hewan Maranatha Bandung dan dilakukan pada Januari–Desember 2020. Hewan coba dibagi menjadi enam kelompok (n=5), yaitu kelompok kontrol, kelompok temulawak, kelompok kunyit, kelompok bawang putih, kelompok bunga delima, dan kelompok  pembanding. Induksi yang diberikan kepada hewan coba adalah pemberian vitamin D3, pakan lemak tinggi, dan propyltiouracil selama 14 hari. Hasil penelitian menunjukkan bahwa pemberian 175 mg/kgBB ekstrak etanol bawang putih (−44,85%), bunga delima (−58,74%), dan rosuvastatin (−40,00%) mengurangi K-LDL dibanding dengan kontrol (p<0,05). Pemberian 175 mg/kgBB ekstrak etanol temulawak (−15,16%), kunyit (−14,02%), bawang putih (−22,80%), bunga delima (−65,24%), dan rosuvastatin (−18.70%) mengurangi K-total dibanding dengan kontrol (p<0,05). Kesimpulannya, bunga bawang putih dan delima menurunkan K-LDL, sedangkan temulawak, kunyit, bawang putih, dan bunga delima menurunkan K-total.


Keywords


Bawang putih; bunga delima; garlic; Javanese ginger; K-LDL; K-total; kunyit; LDL-C; pomegranate flower; temulawak; total-C; turmeric

Full Text:

PDF

References


World Health Organization. Cardiovascular diseases (CVDs) [Internet]. Geneva, Switzerland: World Health Organization; 2021 June 11 [cited 2021 June 30]. Available from: https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds).

Hussain MA, Mamun AA, Peters SA, Woodward M, Huxley RR. The burden of cardiovascular disease attributable to major modifiable risk factors in Indonesia. J Epidemiol. 2016;26(10):515–21.

Shepherd J, Barter P, Carmena R, Deedwania P, Fruchart JC, Haffner S, et al. Effect of lowering LDL cholesterol substantially below currently recommended levels in patients with coronary heart disease and diabetes: the treating to new targets (TNT) study. Diabetes Care. 2006;29(6):1220–6.

Zulkarnain Z, Triyono A, Novianto F. Jamu formula could reduce plasma cholesterol patients with mild hypercholesterolemia. Health Sci J Indones. 2018;9(2):87–92.

Kania A, Kharisma Y, Dewi MK. Bekatul (Oryza sativa L.) menghambar peningkatan kadar kolesterol darah. GMHC. 2014;2(1):34–41.

Karimah F, Achmad S, Suganda RR. Efek jus buah naga super merah (Hylocereus costaricensis) dan simvastatin terhadap kadar kolesterol total darah dan bobot badan tikus jantan galur Wistar hiperkolesterolemia. GMHC. 2014;2(2):79–84.

Prawiradilaga RS, Shahib MN, Farimah SN. Perbedaan efek infusa bubuk kedelai (Glycine max), jamur tiram (Pleurotus ostreatus), dan campuran keduanya terhadap kadar kolestrol LDL, ekspresi gen reseptor LDL hati, dan berat omentum majus mencit model hiperlipidemia. GMHC. 2016;4(1):33–43.

Namazi F, Shomali T, Taghikhani P, Nazifi S. Protective effect of Urtica dioica leaf hydro alcoholic extract against experimentally-induced atherosclerosis in rats. Avicenna J Phytomed. 2018;8(3):254–62.

Alipin K, Tanzania W, Kuntana YP. The effects of temulawak extract and yoghurt on HDL-LDL mice blood exposed waste cooking oil. In: Rahadian R, Aminin ALN, Darmawan A, Astuti Y, Huda MB, editors. Proceedings of 5th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application (5th ISNPINSA); 2015 October 7–8; Semarang, Indonesia. Semarang: Undip Press; 2016. p. 9–11.

Aznam N, Atun S. Pharmacological test of herbal products from temulawak (Curcuma xanthorhiza) as antihypercholesterol by in vivo. Int J Pharmacogn Phytochem Res. 2016;8(5);807–11.

Fikriah I. Effect of curcumin on the levels of total cholesterol, LDL cholesterol, the amount of F2-isoprostan and foam cell in aortic wall of rats with atherogenic diet. FMI. 2007;43(3):136–40.

Hussein SA, El-Senosi YA, Ragab MR, Hammad MMF. Hypolipidemic effect of curcumin in hyper-cholesterolemic rats. Benha Vet Med J. 2014;27(2):277–89.

Sukandar EY, Nurdewi, Elfahmi. Antihypercholesterolemic effect of combination of Guazuma ulmifolia Lamk. leave and Curcuma xanthorrhiza Roxb. rhizomes extract in Wistar rats. Int J Pharmacol. 2012;8(4):277–82.

Jatnika G, Hartanto S. Efforts in hypercholesterolemia treatment using turmeric (curcuminoid) extract phytotherapy on obese patients: preclinical study. JKP. 2018;6(3):227–34.

Budiman I, Tjokropranoto R, Widowati W, Fauziah N, Erawijantari PP. Potency of turmeric (Curcuma longa L.) extract and curcumin as anti-obesity by inhibiting the cholesterol and triglycerides synthesis in HepG2 cells. Int J Res Med Sci. 2015;3(5):1165–71.

Alwi I, Santoso T, Suyono S, Sutrisna B, Suyatna FD, Kresno SB, et al. The effect of curcumin on lipid level in patients with acute coronary syndrome. Acta Med Indones. 2008;40(4):201–10.

Mughal MH. Turmeric polyphenols: a comprehensive review. Integr Food Nutr Metab. 2019;6(5):10.15761/IFNM.1000269.

Yeh YY, Liu L. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: human and animal studies. J Nutr. 2001;13(3)1:989S–93.

Lu Y, He Z, Shen X, Xu X, Jie F, Wu S, et al. Cholesterol-lowering effect of allicin on hypercholesterolemic ICR mice. Oxid Med Cell Longev. 2012:2012:489690.

Sun YE, Wang W, Qin J. Anti-hyperlipidemia of garlic by reducing the level of total cholesterol and low-density lipoprotein: a meta-analysis. Medicine (Baltimore). 2018;97(18):e0255.

Sharifi-Rad J, Silva NCC, Jantwal A, Bhatt ID, Sharopov F, Cho WC et al. Therapeutic potential of allicin-rich garlic preparations: emphasis on clinical evidence toward upcoming drugs formulation. Appl Sci (Basel). 2019;9(24):5555.

Maisaroh S, Zahro C, Puspitasari DR, Wahdi A, Pratiwi TF. Effective consumption of garlic (Allium sativum Linn) on decreasing blood cholesterol levels. IOP Conf Ser Earth Environ Sci. 2020;519:012004.

Anoosh E, Mojtaba E, Fatemeh S. Study the effect of juice of two variety of pomegranate on decreasing plasma LDL cholesterol. Proc Soc Behav Sci. 2010;2(2):620–3.

Resti PV, Utami S, Arsyad. Antioxidant activity potential of red pomegranate (Punica granatum L.) peel as herbal tea. Mutiara Medika. 2020;20(2):79–84.

Wardani PM, Hawa H. A comparison between the effectiveness of the ethanol based extract of pomegranate peel (Punica granatum) and simvastatin drug for lowering blood LDL level in hypercholesterolemic male rats (Rattus novergicus). JKKI. 2015;7(2):46–51.




DOI: https://doi.org/10.29313/gmhc.v9i2.7767

pISSN 2301-9123 | eISSN 2460-5441


Visitor since 19 October 2016: 

View My Stats


Free counters!


This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.