.png)
_(1).png){kind=logo}
Cytotoxicity Effect of Aqueous Propolis Extract of Geniotrigona thoracica Sumatrans on Colo-201 Colon Cancer Cell Line and Senescence Colo-201 Colon Cancer Cell Line Induced by Low-dose Doxorubicin
Abstract
Propolis, a resinous compound honeybees produce, demonstrates an extensive spectrum of powerful biological properties. However, the anti-cancer activity of propolis derived from Geniotrigona thoracica Sumatrans has yet to be reported. Thus, we sought to investigate the cytotoxicity of aqueous propolis extracts from Geniotrigona thoracica Sumatrans against Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin. This study was conducted at the Parasitology Laboratory of Human, Safety, and Environment, Universitas Padjadjaran Bandung from January to May 2024. This study assessed cell viability using the WST-1 test. Non-induced Colo-201 cells were treated with an aqueous extract of propolis (AEP) 100 ppm, or 5-fluorouracil (5-FU) 5 mg/ml as the positive control or water as a vehicle on untreated control. Colo-201 senescence was induced by doxorubicin 0.1 µM for three days. Doxorubicin-induced Colo-201 senescence was then treated with AEP 100 ppm, with 5-FU 5 mg/ml as the positive control, or with the combination of AEP 100 ppm and 5-FU 5 mg/ml, or water as a vehicle on untreated control. The data were analyzed using SPSS version 25.0, a one-way ANOVA, and Tukey’s post hoc test. The results showed that AEP has cancer-killing effects on Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin. AEP-treated Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin viability were significantly reduced to 37.15% and 13.72%, respectively, although slightly higher than those of the 5-FU-treated one at this concentration. There was also a decrease in the cancer-killing effect of 5-FU from 88.55% in non-induced Colo-201 cells to 41.5% in the doxorubicin-induced Colo-201 senescence model. In conclusion, aqueous extract of propolis from Geniotrigona thoracica Sumatrans showed cancer-killing-effects both on the Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin.
Keywords
Full Text:
PDFReferences
Ferlay J, Ervik M, Lam F, Laversanne M, Colombet M, Mery L, et al. Global cancer observatory: cancer today (version 1.1). [Internet]. Lyon, France: International Agency for Research on Cancer; 2024 [cited 2024 Feb 22]. Available from: from: https://gco.iarc.who.int/today.
Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan Republik Indonesia. Laporan nasional Riskesdas 2018. Jakarta: Badan Penelitian dan Pengembangan Kesehatan; 2019.
Ferlay J, Colombet M, Soerjomataram I, Parkin DM, Piñeros M, Znaor A, et al. Cancer statistics for the year 2020: an overview. Int J Cancer. 2021;149(4):778–89.
Hanahan D. Hallmarks of cancer: new dimensions. Cancer Discov. 2022;12(1):31–46.
Sanada F, Taniyama Y, Muratsu J, Otsu R, Shimizu H, Rakugi H, et al. IGF binding protein-5 induces cell senescence. Front Endocrinol (Lausanne). 2018;9:53.
Kciuk M, Gielecińska A, Mujwar S, Kołat D, Kałuzińska-Kołat Ż, Celik I, et al. Doxorubicin-an agent with multiple mechanisms of anticancer activity. Cells. 2023;12(4):659.
Tacar O, Sriamornsak P, Dass CR. Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems. J Pharm Pharmacol. 2013;65(2):157–70.
Guo Y, Tang Y, Lu G, Gu J. p53 at the crossroads between doxorubicin-induced cardiotoxicity and resistance: a nutritional balancing act. Nutrients. 2023;15(10):2259.
Demaria M, O’Leary MN, Chang J, Shao L, Liu S, Alimirah F, et al. Cellular senescence promotes adverse effects of chemotherapy and cancer relapse. Cancer Discov. 2017;7(2):165–76.
Anaka M, Abdel-Rahman O. Managing 5FU cardiotoxicity in colorectal cancer treatment. Cancer Manag Res. 2022;14:273–85.
Forma E, Bryś M. Anticancer activity of propolis and its compounds. Nutrients. 2021;13(8):2594.
Farida S, Pratami DK, Sahlan M, Mun’im A, Djamil R, Winarti W, et al. In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens. J King Saud Univ Sci. 2023;35(6):102738.
Altabbal S, Athamnah K, Rahma A, Wali AF, Eid AH, Iratni R, et al. Propolis: a detailed insight of its anticancer molecular mechanisms. Pharmaceuticals (Basel). 2023;16(3):450.
Hossain R, Quispe C, Khan RA, Saikat ASM, Ray P, Ongalbek D, et al. Propolis: an update on its chemistry and pharmacological applications. Chinese Med. 2022;17(1):100.
Elumalai P, Muninathan N, Megalatha ST, Suresh A, Kumar KS, Jhansi N, et al. An insight into anticancer effect of propolis and its constituents: a review of molecular mechanisms. Evid Based Complement Alternat Med. 2022;2022:5901191.
Mulyati AH, Sulaeman A, Marliyati SA, Rafi M, Fikri AM. Phytochemical analysis and antioxidant activities of ethanol extract of stingless bee propolis from Indonesia. AIP Conf Proc. 2020;2243(1):30014.
Kustiawan PM, Puthong S, Arung ET, Chanchao C. In vitro cytotoxicity of Indonesian stingless bee products against human cancer cell lines. Asian Pac J Trop Biomed. 2014;4(7):549–56.
Kustiawan PM, Phuwapraisirisan P, Puthong S, Palaga T, Arung ET, Chanchao C. Propolis from the stingless bee Trigona incisa from East Kalimantan, Indonesia, induces in vitro cytotoxicity and apoptosis in cancer cell lines. Asian Pac J Cancer Prev. 2015;16(15):6581–9.
Kinasih I, Julita U, Suryani Y, Cahyanto T, Khoirunnisa EM, Putra RE. Comparing the effect of two coating methods of Tetragonula laeviceps propolis water extraction on physical quality and shelf life of eggs kept in room temperature. Malays Appl Biol. 2021;49(2):75–9.
Sarı C, Kolaylı S, Eyüpoğlu FC. A comparative study of MTT and WST-1 assays in cytotoxicity analysis. Haydarpasa Numune Med J. 2021;61(3):281–8.
Kılıç Eren M, Pilevneli H, Ak C, Karlıtepe A, Kaygusuz N. Therapy induced senescence promote expression of death receptors in breast cancer cells. JIAM. 2021;1(1):1–10.
Seyhan MF, Yılmaz E, Timirci-Kahraman Ö, Saygılı N, Kısakesen Hİ, Gazioğlu S, et al. Different propolis samples, phenolic content, and breast cancer cell lines: variable cytotoxicity ranging from ineffective to potent. IUBMB Life. 2019;71(5):619–31.
Utami S, Endrini S, Batubara L, Rahmah NA, Syarif I, Nafik S, et al. Cytotoxicity of combination doxorubicin and Garcinia picrorrhiza fruit extract on fibroblast cell. GMHC. 2023;11(3):195–9.
El-Far AH, Darwish NHE, Mousa SA. Senescent colon and breast cancer cells induced by doxorubicin exhibit enhanced sensitivity to curcumin, caffeine, and thymoquinone. Integr Cancer Ther. 2020;19:1534735419901160.
Utispan K, Chitkul B, Koontongkaew S. Cytotoxic activity of propolis extracts from the stingless bee Trigona sirindhornae against primary and metastatic head and neck cancer cell lines. Asian Pac J Cancer Prev. 2017;18(4):1051–5.
Campos JF, Dos Santos HF, Bonamigo T, de Campos Domingues NL, de Picoli Souza K, Dos Santos EL. Stingless bee propolis: new insights for anticancer drugs. Oxid Med Cell Longev. 2021;2021:2169017.
Kustiawan PM, Lirdprapamongkol K, Palaga T, Puthong S, Phuwapraisirisan P, Svasti J, et al. Molecular mechanism of cardol, isolated from Trigona incisa stingless bee propolis, induced apoptosis in the SW620 human colorectal cancer cell line. BMC Pharmacol Toxicol. 2017;18(1):32.
Amalia E, Diantini A, Subarnas A. Water-soluble propolis and bee pollen of Trigona spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF-7 cell line. Oncol Lett. 2020;20(5):274.
Oršolić N, Jazvinšćak Jembrek M. Molecular and cellular mechanisms of propolis and its polyphenolic compounds against cancer. Int J Mol Sci. 2022;23(18):10479.
DOI: https://doi.org/10.29313/gmhc.v12i2.13760
pISSN 2301-9123 | eISSN 2460-5441
Visitor since 19 October 2016:
_(1).jpg){kind=logo}
Global Medical and Health Communication is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.