Impact of Propolis Administration on Osteocrin Expression and Osteoblast-to-osteoclast Ratio in the Femurs of Rats Fed a High-fat Diet

Alvira Widiyanti, Vita Murniati Tarawan, Leonardo Lubis

Abstract


A high-fat diet (HFD) is associated with bone inflammatory processes that can affect bone remodeling balance. Osteocrin produced by periosteal osteoblasts correlates with osteoblast activity, is expressed on osteoblast-derived cells, and is localized in osteoblasts and young osteocytes. Propolis is an antioxidant and anti-inflammatory in bone remodeling by inhibiting proinflammatory factors NF-κB and COX-2, reducing inflammatory suppression of cytokines responsible for osteoclast differentiation and osteoblast apoptosis. The flavonoid content increases the production of nitric oxide and osteoprotegerin, which enhances osteoblastogenesis. This study determined the effect of propolis administration on bone formation and resorption in bone previously damaged by an HFD. This research was conducted in the Animal Laboratory of Postgraduate Building Dipati Ukur and Genetics and Molecular Laboratory Eycman Building, Faculty of Medicine, Universitas Padjadjaran. The research time was from January 2023–May 2024. Male Wistar rats were divided into four groups: normal chow diet (NCD), NCD with propolis administration, HFD, and HFD with propolis administration. The 12-week-old rats were given an HFD for 12 weeks and then treated with propolis at a 300 mg/kgBW dose for nine weeks. The administration of propolis increased the ratio of osteoblasts-osteoclast cells in the femur of the HFD rats but did not affect periosteal osteocrin expression.

Keywords


Bone; HFD; osteoblast; osteoclast; osteocrin

Full Text:

PDF

References


Wang T, Zhu X, Dai F, Li C, Huang D, Fang Z, et al. Effects of a standard high-fat diet with or without multiple deficiencies on bone parameters in ovariectomized mature rat. PLoS One. 2017;12(9):e0184983.

Zhu R, Wang Z, Xu Y, Wan H, Zhang X, Song M, et al. High-fat diet increases bone loss by inducing ferroptosis in osteoblasts. Stem Cells Int. 2022;2022:9359429.

Duan W, Wang Q, Li F, Xiang C, Zhou L, Xu J, et al. Anti-catabolic effect of caffeic acid phenethyl ester, an active component of honeybee propolis on bone loss in ovariectomized mice: a micro-computed tomography study and histological analysis. Chin Med J (Engl). 2014;127(22):3932–6.

Graham LS, Tintut Y, Parhami F, Kitchen CM, Ivanov Y, Tetradis S, et al. Bone density and hyperlipidemia: the T-lymphocyte connection. J Bone Miner Res. 2010;25(11):2460–9.

Kanai Y, Yasoda A, Mori KP, Watanabe-Takano H, Nagai-Okatani C, Yamashita Y, et al. Circulating osteocrin stimulates bone growth by limiting C-type natriuretic peptide clearance. J Clin Invest. 2017;127(11):4136–47.

Bord S, Ireland DC, Moffatt P, Thomas GP, Compston JE. Characterization of osteocrin expression in human bone. J Histochem Cytochem. 2005;53(10):1181–7.

Hossain R, Quispe C, Khan RA, Saikat ASM, Ray P, Ongalbek D, et al. Propolis: an update on its chemistry and pharmacological applications. Chin Med. 2022;17(1):100.

Farida S, Pratami DK, Sahlan M, Laksmitawati DR, Rohmatin E, Situmorang H. In-vitro antioxidant, in-vivo anti-inflammatory, and acute toxicity study of Indonesian propolis capsule from Tetragonula sapiens. Saudi J Biol Sci. 2022;29(4):2489–500.

Segueni N, Zellagui A, Moussaoui F, Lahouel M, Rhouati S. Flavonoids from Algerian propolis. Arab J Chem. 2016;9(Suppl 1):S425–8.

Minematsu A, Nishii Y, Sakata S. High-fat/high-sucrose diet results in higher bone mass in aged rats. Bone Rep. 2018;8:18–24.

Mazur CM, Castro Andrade CD, Tokavanich N, Sato T, Bruce M, Brooks DJ, et al. Partial prevention of glucocorticoid-induced osteocyte deterioration in young male mice with osteocrin gene therapy. iScience. 2022;25(9):105019.

Ekeuku SO, Chin K. Application of propolis in protecting skeletal and periodontal health—a systematic review. Molecules. 2021;26(11):3156.

Darmadi D, Mustamsir E. The effect of propolis on increasing the number of osteoblasts and chondrocytes, and decreasing the number of osteoclasts in Wistar rats (Rattus novergicus) with femoral bone fracture. IOSR J Dent Med Sci. 2016;15(12):90–5.

Juwita DA, Ahmadi A, Rachmaini F, Abdillah R, Fatma RM. Effect of propolis on bone quality and cortical bone thickness of ovariectomized female Wistar white rats as a model for osteoporosis. Pharm Sci Res. 2021;8(3):121–7.

Zheng Y, Wu Y, Tao L, Chen X, Jones TJ, Wang K, et al. Chinese propolis prevents obesity and metabolism syndromes induced by a high fat diet and accompanied by an altered gut microbiota structure in mice. Nutrients. 2020;12(4):959.

Duan Y, Zeng L, Zheng C, Song B, Li F, Kong X, et al. Inflammatory links between high fat diets and diseases. Front Immunol. 2018;9:2649.

Moore KL, Dalley AF, Agur AMR. Clinically oriented anatomy. 7th edition. Baltimore: Lippincott Williams & Wilkins; 2014.

Watanabe-Takano H, Ochi H, Chiba A, Matsuo A, Kanai Y, Fukuhara S, et al. Mechanical load regulates bone growth via periosteal osteocrin. Cell Rep. 2021;36(2):109380.

McWain MA, Pace RL, Nalan PA, Lester DB. Age-dependent effects of social isolation on mesolimbic dopamine release. Exp Brain Res. 2022;240(10):2803–15.

Nguyen-Yamamoto L, Tanaka KI, St-Arnaud R, Goltzman D. Vitamin D-regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification. JCI Insight. 2019;4(13):e126467.




DOI: https://doi.org/10.29313/gmhc.v12i2.13833

pISSN 2301-9123 | eISSN 2460-5441


Visitor since 19 October 2016: 


Free counters!


Global Medical and Health Communication is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.