EVALUASI INVITRO AKTIVITAS ANTIOKSIDAN DAN TABIR SURYA DARI EKSTRAK DAUN DAN AKAR TUMBUHAN Elephantopus mollis Kunth

Verawati Verawati; Miftahur Rahmi; Intan Suci Mayasari

doi:10.29313/jiff.v5i2.8361

EVALUASI INVITRO AKTIVITAS ANTIOKSIDAN DAN TABIR SURYA DARI EKSTRAK DAUN DAN AKAR TUMBUHAN Elephantopus mollis Kunth

Verawati Verawati, Miftahur Rahmi, Intan Suci Mayasari

Abstract

Solar radiation in Indonesia is quite abundant because geographically it is located at equatorial latitudes, so people are vulnerable to feel the bad effects of solar radiation. Chemical components with antioxidant properties can also act as sunscreens to prevent the negative effects of solar radiation. This study aimed to determine the antioxidant and sunscreen activity of the leaf and root extract of the Tutup Bumi (Elephantopus mollis Kunth) plant. The antioxidant activity test was examined by the DPPH radical scavenging method (2,2-diphenyl-1-picrylhydrazyl) while the sunscreen potency was determined by calculating the SPF value using the Mansur method spectrophotometrically. The results of the examination showed that the antioxidant activity was quite strong, namely the leaf extract with IC50 79.38 g/mL and root extract 78.38 g/mL. The SPF value of the leaf extract is better than that of the root extract, where at a concentration of 500 ppm, the leaf extract has an SPF of 7.661 in the Extra category while the root extract at the same concentration has an SPF value of 5.176 in the Medium category. Tutup Bumi leaf and root extracts have potential as antioxidants and sunscreens.

Keywords

tutup bumi; elephantopus mollis; antioxidant; SPF

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References

Bessada, S. M. F., Barreira, J. C. M., & Oliveira, M. B. P. P. (2015). Asteraceae species with most prominent bioactivity and their potential applications: A review. Industrial Crops and Products, 76, 604–615. https://doi.org/10.1016/j.indcrop.2015.07.073

But, P. P. H., Hon, P. M., Cao, H., & Che, C. T. (1996). A new sesquiterpene lactone from Elephantopus mollis. Planta Medica, 62(5), 474–476. https://doi.org/10.1055/s-2006-957945

Dutra, E. A., Da Costa E Oliveira, D. A. G., Kedor-Hackmann, E. R. M., & Miritello Santoro, M. I. R. (2004). Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Revista Brasileira de Ciencias Farmaceuticas/Brazilian Journal of Pharmaceutical Sciences, 40(3), 381–385. https://doi.org/10.1590/S1516-93322004000300014

Ebrahimzadeh, M. A., Enayatifard, R., Khalili, M., Ghaffarloo, M., Saeedi, M., & Charati, J. Y. (2014). Correlation between sun protection factor and antioxidant activity, phenol and flavonoid contents of some medicinal plants. Iranian Journal of Pharmaceutical Research, 13(3), 1041–1048. https://doi.org/10.22037/ijpr.2014.1554

Empinotti dkk. (2008). Artigo Estudo anatômico de folha e caule de Elephantopus mollis Kunth ( Asteraceae ). 18(1), 108–116.

Gachet, M. S., Lecaro, J. S., Kaiser, M., Brun, R., Navarrete, H., Muñoz, R. A., Schühly, W. (2010). Assessment of anti-protozoal activity of plants traditionally used in Ecuador in the treatment of leishmaniasis. Journal of Ethnopharmacology, 128(1), 184–197. https://doi.org/https://doi.org/10.1016/j.jep.2010.01.007

Molyneux, P. (2004). The use of the stable free radical diphenylpicryl- hydrazyl ( DPPH ) for estimating antioxidant activity. Songklanakarin J. Sci. Technol., 26(2), 211–219.

Ooi, K. L., Muhammad, T. S. T., Tan, M. L., & Sulaiman, S. F. (2011). Cytotoxic, apoptotic and anti-α-glucosidase activities of 3,4-di-O-caffeoyl quinic acid, an antioxidant isolated from the polyphenolic-rich extract of Elephantopus mollis Kunth. Journal of Ethnopharmacology, 135(3), 685–695. https://doi.org/10.1016/j.jep.2011.04.001

Phongpaichit, S., Nikom, J., Rungjindamai, N., Sakayaroj, J., Hutadilok-Towatana, N., Rukachaisirikul, V., & Kirtikara, K. (2007). Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunology and Medical Microbiology, 51(3), 517–525. https://doi.org/10.1111/j.1574-695X.2007.00331.x

Ragasa, C. Y., Alimboyoguen, A. B., & Shen, C.-C. (2009). Antimicrobial terpenoids from Elephantopus mollis. NRCP Research Journal, 10(1), 33–38.

ROBERT M. SAYRE,* PATRICIA POH AGIN, G. J. L. and E. M., & Dept. (1979). a Comparison of I N V I V O and I N Vitro Testing of Sunscreening Formulas. Photochemistry and Photobiology, 29, 559–566.

Schalka, S., & Manoel, V. (2011). Sun protection factor : meaning and controversies Fator de proteção solar : significado e controvérsia. Anais Brasileiros Dermatologia, 86(3), 507–515.

DOI: https://doi.org/10.29313/jiff.v5i2.8361